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US 2010/0247570 A1Published application
High Mannose Glycoprotein Epitopes
AssigneeProcell, Inc.
InventorsYvonne Rosenberg, Markus Sack, Rainer Fischer
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Abstract
Glycoproteins containing high mannose oligosaccharides are obtained and used to generate antibody to the mannose portion of the molecule. Such antibodies can be used in a diagnostic assay. The glycoproteins can be used to generate neutralizing antibody.
Claims21
A composition comprising an isolated recombinant glycoprotein comprising an immunogenic high mannose epitope and a pharmaceutically acceptable carrier.
A method of making a pharmaceutically administrable glycoprotein comprising an immunogenic high mannose epitope, comprising:
- (a) obtaining a cell transformed to express a polypeptide comprising a KDEL tetrapeptide at the C terminus thereof;
- (b) culturing said cell of step (a);
- (c) isolating high mannose glycoproteins from said cell of step (b);
- and
- (d) mixing said glycoprotein of step (c) comprising an immunogenic high mannose epitope with a pharmaceutically acceptable carrier, excipient or diluent to yield said administrable glycoprotein.
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Dependencies are inferred from the claim text.