Image processing device, imaging device, computer-readable storage medium, and image processing method
AssigneeOlympus Corporation
InventorKeiji HIGUCHI
Life of this patent
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Abstract
A reflected light image and a fluorescent light image of an observed region are acquired with an imaging system. A gradation level of the reflected light image is set on the basis of a feature value of the acquired reflected light image. The focus of the imaging system is controlled in accordance with the set gradation level. The fluorescent light image is corrected using a focus-controlled reflected light image of the observed region. Accordingly, the image of an object in the observed region from which the fluorescent light is generated is sharpened.
Claims14
An image processing device comprising:
- an image acquiring unit that acquires a reflected light image and a fluorescent light image of an observed region, the reflected and the fluorescent light images being captured by an imaging system;
- a feature value calculating unit that calculates a feature value of the reflected light image acquired by the image acquiring unit;
- a gradation level setting unit that sets a gradation level of the reflected light image on the basis of the feature value;
- a control unit that controls a focus of the imaging system in accordance with the gradation level;
- and
- a corrected fluorescent light image generating unit that generates a corrected fluorescent light image that is obtained by correcting the fluorescent light image using a focus-controlled reflected light image of the observed region that is acquired by the image acquiring unit after the focus is controlled.
An imaging device, comprising:
- a light source unit that applies illuminating light and excitation light to an observed region;
- an imaging system that captures a reflected light image of the observed region based on the illuminating light that is reflected from the observed region, and captures a fluorescent light image of the observed region based on fluorescent light that is generated from the observed region due to application of the excitation light;
- a feature value calculating unit that calculates a feature value of the reflected light image that is captured by the imaging system;
- a gradation level setting unit that sets a gradation level of the reflected light image on the basis of the feature value;
- a control unit that controls the focus of the imaging system in accordance with the gradation level;
- and
- a corrected fluorescent light image generating unit that generates a corrected fluorescent light image that is obtained by correcting the fluorescent light image using a focus-controlled reflected light image of the observed region that is acquired by the image acquiring unit after the focus is controlled.
A computer-readable storage medium that stores therein an image processing program that contains instructions for causing a computer to perform:
- acquiring a reflected light image of an observed region based on light that is reflected from the observed region;
- calculating a feature value of the reflected light image;
- setting a gradation level of the reflected light image on the basis of the feature mount;
- controlling a focus of an imaging system for the reflected light image in accordance with the gradation level;
- acquiring a focus-controlled reflected light image of the observed region after the focus is controlled;
- and
- generating a corrected fluorescent light image that is obtained by correcting the fluorescent light image of the observed region, using the focus-controlled reflected light image.
An image processing method, comprising:
- acquiring a reflected light image of an observed region based on light that is reflected from the observed region;
- calculating a feature value of the reflected light image;
- setting a gradation level of the reflected light image on the basis of the feature mount;
- controlling a focus of an imaging system for the reflected light image in accordance with the gradation level;
- acquiring a focus-controlled reflected light image of the observed region after the focus is controlled;
- and
- generating a corrected fluorescent light image that is obtained by correcting the fluorescent light image of the observed region, using the focus-controlled reflected light image.
Dependencies are inferred from the claim text.