Rna constructs
AssigneeDevgen NV
InventorsMarc Van De Craen, Geert Karel Maria Plaetinck, Isabelle Vercauteren, Marc Georges Logghe, Thierry Andre Olivier Eddy Bogaert, Richard Zwaal
Life of this patent
Estimating the term…
Abstract
The present invention concerns concatemer and/or stabilized RNA constructs capable of forming dsRNA, optionally comprising a sequence capable of protecting the dsRNA against RNA processing in a host cell. The invention also relates to methods of producing these constructs and to methods for using these constructs. The constructs according to the present invention are particularly useful in plant pest control.
Claims53
An isolated nucleic acid encoding a double-stranded ribonucleic acid (dsRNA) effective in RNAi gene silencing, wherein the dsRNA comprises at least two dsRNA fragments, each fragment comprising annealed complementary strands, one of which is complementary to at least part of the nucleotide sequence of a target sequence.
An isolated nucleic acid encoding a dsRNA comprising at least one dsRNA fragment, wherein the dsRNA comprises annealed complementary strands, one of which is complementary to at least part of the nucleotide sequence of a target sequence, which nucleic acid further encodes at least one RNA sequence that protects the dsRNA against RNA processing.
(canceled)
(canceled)
A method for controlling pest growth on a cell or an organism or for preventing pest infestation of a cell or an organism susceptible to infection by said pest species, comprising contacting said pest species with a double-stranded RNA or RNA construct of claim 52, whereby the double-stranded RNA or RNA construct is taken up by said pest species and thereby controls pest growth or prevents pest infestation.
A method for down-regulating expression of at least one target gene in a pest species, comprising contacting said pest species with a double-stranded RNA or RNA construct of claim 52, whereby the double-stranded RNA or RNA construct is taken up by the pest species and thereby down-regulates expression of the pest target gene(s).
(canceled)
A kit comprising a double stranded RNA of claim 52 and instructions for use of the said double stranded RNA, RNA construct, nucleotide sequence, recombinant DNA, cell or composition for treating pest infection of plants.
Dependencies are inferred from the claim text.