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US 2010/0169997 A1Published application
Promotion of somatic embryogenesis in plants by wuschel gene expression
AssigneeThe Rockefeller University
InventorsJianru ZUO, Qi-Wen NIU, Giovanna FRUGIS, Nam-Hai CHUA
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Abstract
The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.
Claims8
A method for producing a haploid plant comprising
- (a) stably transforming a plant cell with a DNA molecule comprising at least one Wuschel coding sequence under the control of a promoter to produce a transgenic plant cell, wherein the promoter is selected from the group consisting of a haploid tissue specific promoter, an inducible promoter and a promoter that is both haploid-tissue specific and inducible, wherein the Wuschel coding sequence is SEQ ID NO:9,
- (b) generating a transgenic plant from said transgenic plant cell,
- (c) overexpressing the Wuschel coding sequence in a haploid tissue of said transgenic plant to produce a haploid somatic embryo,
- (d) growing said embryo into a haploid plant.
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Dependencies are inferred from the claim text.