Conditional Mst Overexpressing Construct and Conditional Myostatin Overexpressing Transgenic Mouse
AssigneeCharles Drew University of Medicine and Science
InventorSuzanne Porszasz-Reisz
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Abstract
Provided herein are novel nucleic acid sequences, vectors comprising such nucleic acid sequences, host cells comprising such vectors, and transgenic animals comprising such nucleic acid sequences, and related molecules and methods relating thereto. Such novel nucleic acid sequences, vectors comprising such nucleic acid sequences, host cells comprising such vectors, and transgenic animals comprising such nucleic acid sequences, and related molecules and methods provide conditional overexpression of genes, such as myostatin, and transgenic animals conditionally overexpression genes, such as myostatin.
Claims22
A conditional bicistronic myostatin expression construct comprising a construct selected from the group of constructs consisting of polyA-EGFP/IRES-Mst/HA-TRE//MCK-rtTA/BFP-polyA, polyA-BFP/rtTA-MCK//TRE-HA/Mst-IRES/EGFP-polyA, TRE-HA/Mst-IRES/EGFP-polyA//MCK-rtTA/BFP-polyA, MCK-rtTA/BFP-polyA//TRE-HA/Mst-IRES/EGFP-polyA, and polyA-EGFP/IRES-Mst/HA-TRE//MCK-rtTA/BFP-polyA, where MCK is a promoter selected from MCK and MCK-3E.
A transgenic non-human animal produced by:
- (a) crossing a first transgenic non-human animal comprising a first (regulatory) construct that expresses rtTA operably linked to promoter MCK or MCK-3E with a second non-human animal comprising a second (response) construct comprising Mst or Mst cDNA operably linked to a promoter TRE and (b) selecting from the first generation offspring a transgenic non-human animal having a genome comprising both the first regulatory construct and the second response construct.
Dependencies are inferred from the claim text.