Loading patent…
AssigneeMetabolix, Inc.
InventorsWilliam R. Farmer, Jeff Bickmeier, Chenfeng Lu, Dong-Eun Chang, Frank Skraly, Thomas Martin Ramseier
Estimating the term…
Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is E. coli. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).
A recombinant organism genetically engineered to convert glutarate semialdehyde or 5-aminopentanoate into a 5 carbon monomer, polymer or copolymer thereof, wherein the recombinant organism expresses at least one or more gene(s) encoding one or more enzymes selected from the group consisting of:
A recombinant organism for producing polymers from lysine wherein the recombinant organism is genetically engineered to express at least one enzyme selected from the group consisting of lysine 2-monooxygenase, EC 1.13.12.2;
A recombinant organism for producing 1,5-pentanediol from lysine wherein the recombinant organism is genetically engineered to express at least one enzyme selected from the group consisting of lysine 2-monooxygenase, EC 1.13.12.2;
1,5 Pentanediol produced from renewable carbon substrate.
Dependencies are inferred from the claim text.