Production of peracids using an enzyme having perhydrolysis activity

A process to disinfect a hard surface or inanimate object using an enzymatically produced peroxycarboxylic acid composition, said process comprising:

• a) providing a set of reaction components comprising:
• 1) at least one substrate selected from the group consisting of:
• i) esters having the structure
• [X]mR5
• wherein X=an ester group of the formula R6C(O)O;
• R6=C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R6 optionally comprises one or more ether linkages for R6=C2 to C7;
• R5=a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety optionally substituted with hydroxyl groups;
• wherein each carbon atom in R5 individually comprises no more than one hydroxyl group or no more than one ester group;
• wherein R5 optionally comprises one or more ether linkages;
• m=1 to the number of carbon atoms in R5;
• and
• wherein said esters have a solubility in water of at least 5 ppm at 25° C.;
• ii) glycerides having the structure
• wherein R1=C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
• and
• iii) acetylated saccharides selected from the group consisting of acetylated monosaccharides, acetylated disaccharides, and acetylated polysaccharides;
• 2) a source of peroxygen;
• and
• 3) an enzyme catalyst comprising an enzyme, wherein said enzyme has perhydrolase activity and comprises a polypeptide having at least 95% identity to the amino acid sequence set forth in SEQ ID NO:90;
• b) combining said reaction components under suitable aqueous reaction conditions whereby a peroxycarboxylic acid is produced;
• and
• c) contacting said hard surface or inanimate object with the peroxycarboxylic acid whereby said surface or said inanimate object is disinfected.

A process to disinfect a hard surface or inanimate object using an enzymatically produced peroxycarboxylic acid composition, said process comprising combining on said hard surface or inanimate object under suitable aqueous reaction conditions a set of reaction components comprising:

• 1) at least one substrate selected from the group consisting of:
• i) esters having the structure
• [X]mR5
• wherein X=an ester group of the formula R6C(O)O;
• R6=C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R6 optionally comprises one or more ether linkages for R6=C2 to C7;
• R5=a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety optionally substituted with hydroxyl groups;
• wherein each carbon atom in R5 individually comprises no more than one hydroxyl group or no more than one ester group;
• wherein R5 optionally comprises one or more ether linkages;
• m=1 to the number of carbon atoms in R5;
• and
• wherein said esters have a solubility in water of at least 5 ppm at 25° C.;
• ii) glycerides having the structure
• wherein R1=C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R3 and R4 are individually H or R1C(O);
• and
• iii) acetylated saccharides selected from the group consisting of acetylated monosaccharides, acetylated disaccharides, and acetylated polysaccharides;
• 2) a source of peroxygen;
• and
• 3) an enzyme catalyst comprising an enzyme wherein said enzyme has perhydrolase activity and comprises a polypeptide having at least 95% identity to the amino acid sequence set forth in SEQ ID NO:90;
• thereby generating peroxycarboxylic acid, whereby said hard surface or inanimate object is disinfected.