Protein-coding RNA to correct mitochondrial dysfunction
AssigneeCouncil of Scientific and Industrial Research
InventorSamit Adhya
Life of this patent
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Abstract
The present invention provides compositions and methods for using correctional RNA, with a proteinaceous carrier, to stimulate respiration of the cells, tissues, organs or the whole organism of normal or diseased subjects. In one embodiment of the invention, the signal-tagged correctional RNA is one or more protein-coding RNAs (pcRNA) that encode one or more of human mitochondrial proteins. The invention specifically provides a broad-spectrum formulation applicable to a wide variety of disorders that are associated with mitochondrial mutations.
Claims25
A recombinant polynucleotide segment comprising a sequence set forth in SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:11, wherein the sequence comprises an operably linked signal tag.
A recombinant polynucleotide comprising a sequence set forth in SEQ ID NO:12 and a complementary strand thereof selected from the group consisting of SEQ ID NO:13 and SEQ ID NO:14.
A composition comprising a polyribonucleotide sequence set forth in SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:11 or a mixture thereof.
Use of composition comprising an amount of a polyribonucleotide sequence set forth in SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:11 or a mixture thereof, a carrier comprising a group of proteins comprising SEQ ID NOS:
- 1, 3, 5, 6, 7 and 8 and a binding buffer in the preparation of a medicament for the treatment of the disease or physiological condition associated with mitochondrial dysfunction.
A method of treating a disease or physiological condition associated with mitochondrial dysfunction in a mammal comprising administering a composition comprising an amount of a polyribonucleotide sequence set forth in SEQ ID NO:9, SEQ ID NO:10 or SEQ ID NO:11 or a mixture thereof, a carrier comprising a group of proteins comprising SEQ ID NOS:
- 1, 3, 5, 6, 7 and 8 and a binding buffer sufficient for the treatment of the disease or physiological condition associated with mitochondrial dysfunction.
A diagnostic kit for screening mitochondrial dysfunction in a patient sample comprising:
- a carrier (component A), wherein the carrier is subunit R6 (SEQ ID NOS:
- 1, 3, 5, 6, 7 and 8) and/or R8 (SEQ ID NOS:
- 1-8),
- pc RNA (protein-coding RNA) (component B), wherein the pc RNA is a recombinant polyribonucleotide segments comprising a sequence set forth in SEQ ID NO:9, SEQ ID NO:10 and/or SEQ ID NO:11, a binding buffer and an instruction manual for carrying out diagnosis.
Dependencies are inferred from the claim text.