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AssigneeSloan-Kettering Institute for Cancer Research
InventorsOwen A. O'Connor, Francis Sirotnak
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The present invention relates to a method for assessing the sensitivity of a patient's cancer to treatment with 10-propargyl-10-deazaminopterin and a method for selecting a patient for treatment of cancer with 10-propargyl-10-deazaminopterin, by determining the amount of a selected polypeptide expressed by the cancer and comparing the amount with the amount of the selected polypeptide expressed by a reference cancer, wherein the polypeptide includes a member of folate pathways within cells and may include at least one of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT). The present invention also relates to the use of 10-propargyl-10-deazaminopterin in the treatment of multiple myeloma.
A method of selecting a patient for treatment of a cancer with 10-propargyl-10-deazaminopterin, the method comprising the steps of:
A method for assessing sensitivity of a patient's cancer to treatment with 10-propargyl-10-deazaminopterin comprising the steps of:
A method for the treatment of multiple myeloma comprising administering to a patient diagnosed with having multiple myeloma a pharmaceutically acceptable composition comprising a therapeutically effective amount of 10-propargyl-10-deazaminopterin.
A method to modulate the expression of a polypeptide in a patient's cancer comprising administering to the patient an effective amount of 10-propargyl-10-deazaminopterin, wherein the polypeptide is selected from the group consisting of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT).
A kit for assessing sensitivity of a patient's cancer to treatment with 10-propargyl-10-deazaminopterin comprising at least two sets of selected polypeptide RNA-specific primers wherein each set of specific primers produces double stranded DNA complementary to at least one selected polypeptides, wherein each first primers of said sets contains a sequence which can selectively hybridize to RNA, cDNA or an EST complementary to one of the selected polypeptides to create an extension product and each said second primers of said sets is capable of selectively hybridizing to said extension product, wherein the at least one selected polypeptide is selected from the group consisting of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT).
Dependencies are inferred from the claim text.