Altered Mitochondrial Activity in Diseases Resulting From Oxidative Stress
OwnerAfshan Malikinventor
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Abstract
The present invention relates to the identification of a subject having or at risk of developing a disease resulting from oxidative stress in the body. The present invention also relates to the identification of a diabetic subject having or at risk of developing microvascular disease, kidney disease, and/or cardiovascular disease.
Claims41
A method for identifying a subject having or at risk of developing one or more diseases resulting from oxidative stress comprising the steps of:
- (a) providing a test sample from said subject;
- and
- (b) measuring at least one indicator of altered mitochondrial activity in the test sample,
- wherein a difference in mitochondrial activity in the test sample as compared to a control is indicative that said subject has or is at risk of developing said disease(s).
A method for measuring mitochondrial copy number n a cell comprising the steps of:
- (a) amplifying a single copy region of the nuclear genome;
- (b) amplifying a region of the mitochondrial genome;
- (c) quantifying the amount of amplification product from step (a) and step (b);
- and
- (d) calculating the mitochondrial copy number in the cell.
An assay method for identifying one or more agents that modulate one or more diseases resulting from oxidative stress in a subject, comprising the steps of:
- (a) providing a sample comprising one or more mitochondria from the subject;
- (b) contacting said sample with one or more agents;
- and
- (c) measuring the activity of the mitochondria in the sample in the presence and absence of said agent(s),
- wherein a difference between a) the mitochondrial activity in the presence of the agent and b) the mitochondrial activity in the absence of the agent(s) is indicative that the agent(s) can modulate the one or more disease(s) in the subject.
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A method for identifying one or more genes that are involved in the presentation of one or more diseases resulting from oxidative stress, comprising the step of:
- (a) providing a test sample from a subject suffering from one or more diseases resulting from oxidative stress;
- and
- (b) determining the expression profile of one or more mitochondrial genes in said test sample, wherein a difference in the expression profile of one or more mitochondrial genes in said test sample as compared to a sample from a control subject is indicative that said gene(s) are involved in the presentation of said disease(s) in a subject.
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A method of determining oxidative stress in a sample, comprising the step of:
- measuring at least one indicator of altered mitochondrial activity in the test sample, wherein a difference in mitochondrial activity in the test sample as compared to a control is indicative of oxidative stress.
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Dependencies are inferred from the claim text.