Peptides comprising aromatic d-amino acids and methods of use
AssigneeBio Science International, Inc.
InventorByron E. ANDERSON
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Abstract
Disclosed are D-peptides and libraries of D-peptides comprising aromatic D-amino acids. Also disclosed are methods for identifying small D-peptides comprising aromatic D-amino acids that bind to proteins of interest
Claims16
Combinatorial libraries comprising a plurality of D-peptides, wherein each D-peptide comprises from three to seven D-amino acid residues, wherein at least 68% of the D-peptide sequences comprise at least three amino acid residues independently selected from the group consisting of D-tryptophan, D-tyrosine, and D-phenylalanine.
A method for reducing the toxicity of a toxin in a mammal exposed to the toxin comprising delivering to the mammal a D-peptide(s) that binds to the toxin in an amount effective to reduce toxicity, wherein the D-peptide(s) comprises from three to seven D-amino acid residues, wherein at least three of the D-amino residues are independently selected from the group consisting of D-phenylalanine, D-tryptophan, and D-tyrosine.
A method of reducing binding of TNFα to a TNFα receptor comprising delivering to the mammal a D-peptide comprising a pentapeptide core selected from the group consisting of FFFXaa1F, YFXaa1FF, YFYFXaa1, YWXaa1FF, WXaa1YXaa2F, WXaa1YFXaa2 and WXaa1FFXaa2 wherein Xaa1 and Xaa2 are amino acids of the D- or L-configuration independently selected from the group consisting of D, E, K, R, H, N, Q, C, S, T, G, A, V, L, I, M and P.
A method of reducing the binding of TGFβ1 to a TNFβ1 receptor comprising delivering to the mammal a D-peptide comprising a pentapeptide core selected from the group consisting of FFFXaa1W, FWFXaa1Xaa2, FYXaa1YF, FWXaa1Xaa2Xaa3, FXaa1YYW, FXaa1YYXaa2, FWXaa1WY, FFWYW, FXaa1Xaa2FXaa3, FYWXaa1Y, FYWXaa1W, FXaa1YFXaa2, FYYYXaa1, FWXaa1FF and FFXaa1WW wherein Xaa1, Xaa2 and Xaa3 are amino acids of the D- or L-configuration independently selected from the group consisting of D, E, K, R, H, N, Q, C, S, T, G, A, V, L, I, M and P.
A method of reducing inhibiting anti-Ley/H antibody binding to an Ley/H epitope comprising delivering to the mammal a D-peptide comprising a pentapeptide core selected from the group consisting of YYXaa1YY wherein Xaa1 is independently selected from a group consisting of D, E, K, R, H, N, Q, C, S, T, G, A, V, L, I, M and P, the latter amino acids being of D- or L-configuration.
A method of reducing binding of an anti-αGal antibody to an αGal epitope comprising delivering to the mammal a D-peptide comprising a pentapeptide core selected from the group consisting of Xaa1Xaa2WXaa3Y, FFWXaa1Y and FXaa1WXaa2Xaa.3 wherein Xaa1, Xaa2 and Xaa3 are amino acids of the D- or L-configuration independently selected from the group consisting of D, E, K, R, H, N, Q, C, S, T, G, A, V, L, I, M and P.
A D-peptide comprising a sequence of from three to seven D-amino acids, wherein at least three of the amino acid residues are independently selected from the group consisting of D-tryptophan, D-tyrosine, and D-phenylalanine.
Dependencies are inferred from the claim text.