Use of In-Vitro Culture to Design or Test Personalized Treatment Regimens

A method of providing a custom treatment for a subject, the method comprising:

• obtaining a stem cell sample from the subject;
• culturing the sample;
• exposing the sample to an agent;
• evaluating the sample in the presence of and in the absence of the agent to create a first sample profile;
• comparing the first sample profile to a first reference profile indicative of non-responsiveness to the agent, wherein the similarity between the first sample profile and the first reference profile indicates the subject's non-responsiveness to the agent;
• designing a custom compound and exposing the sample to the custom compound;
• evaluating the sample in the presence of and in the absence of the custom compound to create a second sample profile;
• comparing the second sample profile to a second reference profile indicative of responsiveness to the agent;
• wherein the similarity between the second sample profile and the second reference profile indicates the subject's responsiveness to the custom compound;
• and
• wherein the custom compound is provided to the subject as a custom treatment.

A method of screening a custom compound for efficacy, safety, or lack of adverse effect, the method comprising:

• obtaining a stem cell sample from a subject;
• culturing the sample;
• exposing the sample to a custom compound;
• evaluating the sample in the presence of and in the absence of the custom compound to create a sample profile;
• comparing the sample profile to a reference profile generated by an agent which exhibits efficacy, safety, or lack of adverse effect;
• wherein the similarity between the sample profile and the reference profile indicates the efficacy, safety, or lack of adverse effect of the custom compound.

A method of treating a subject in need thereof, the method comprising:

• obtaining a stem cell sample from the subject;
• culturing the sample;
• exposing the sample to an agent;
• evaluating the sample in the presence of and in the absence of the agent to create a first sample profile;
• comparing the first sample profile to a first reference profile indicative of non-responsiveness to the agent, wherein the similarity between the first sample profile and the first reference profile indicates the subject's non-responsiveness to the agent;
• designing a custom compound and exposing the sample to the custom compound;
• evaluating the sample in the presence of and in the absence of the custom compound to create a second sample profile;
• comparing the second sample profile to a second reference profile indicative of responsiveness to the agent;
• wherein the similarity between the second sample profile and the second reference profile indicates the subject's responsiveness to the custom compound;
• and
• wherein the custom compound is provided to the subject as a custom treatment.

A method of treating a subject in need thereof, the method comprising:

• obtaining a stem cell sample from the subject;
• culturing the sample;
• exposing the sample to an agent;
• evaluating the sample in the presence of and in the absence of the agent to create a sample profile;
• comparing the sample profile to a reference profile indicative of responsiveness to the agent;
• wherein the similarity between the sample profile and the reference profile indicates the subject's responsiveness to the agent;
• and
• treating the subject with the agent.

A computer-aided system comprising:

• (a) a computer readable medium comprising computer-executable instructions configured to predict responsiveness of a subject to treatment with an agent;
• (b) input data from:
• (i) a subject profile;
• and (ii) a reference profile;
• and
• (c) an algorithm capable of comparing the data;
• wherein the system outputs a predicted responsiveness by the subject to the agent.