Suppression of secondary capture in microarray assays
OwnerThomas Albert, Jeff Jeddeloh +1inventors
InventorsThomas Albert, Jeff Jeddeloh, Bradley Swanson
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Abstract
The present invention provides for compositions, methods and systems for targeted sequence enrichment. In particular, the present invention provides for enriching for targeted nucleic acid sequences during hybridizations in microarray assays by suppressing secondary capture of non-target nucleic acid sequences.
Claims15
A method of suppressing secondary capture in a nucleic acid hybridization comprising the steps of:
- a) immobilizing one or more nucleic acid probes to capture target nucleic acid sequences in a sample,
- b) adding a secondary capture suppressing agent to said sample wherein said secondary capture suppressing agent comprises a target nucleic acid species specific C0t-1 DNA and a hybridization blocking oligonucleotide, and
- c) applying said sample and said secondary capture suppressing agent to said probes for hybridization to said target sequences.
A method of suppressing secondary capture in a nucleic acid hybridization comprising the steps of:
- a) immobilizing one or more nucleic acid probes to capture target nucleic acid sequences in a sample,
- b) adding a secondary capture suppressing agent to said sample wherein said secondary capture suppressing agent comprises a hybridization blocking oligonucleotide which is complementary to adapter ligated nucleic acids, and
- c) applying said sample and said secondary capture suppressing agent to said probes for hybridization to said target sequences.
A composition comprising species specific C0t-1 DNA and hybridization blocking oligonucleotides wherein said composition is used in blocking secondary capture in hybridization based assays.
Dependencies are inferred from the claim text.