Microarray chip and method of fabricating for the same
AssigneeNational Health Research Institute
InventorsJyh-Lyh Juang, Yi-You Huang, Po-Cheng Chen
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Abstract
The present invention provides a microarray chip for use in the analysis of various sample types. The microarray chips disclosed herein generally comprise a substrate covered with a coating material comprising a photoresist material, wherein the coating material is patterned to comprise a plurality of microstructures such as microwells and/or microcolumns. Methods for preparing and utilizing the microarray chips of the invention are further provided. The microarray chips of the instant invention find particular use in high-throughput assays.
Claims20
A microarray chip comprising:
- (a) a substrate;
- and
- (b) a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microstructures on the substrate.
A platform for analyzing binding of a probe to a sample comprising:
- (a) an array chip comprising at least one substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells;
- (b) a means for applying the probe to the sample in the microwells;
- and
- (c) a means for detecting any binding of the probe and the sample and any phenotypic change resulting from binding of the probe and the sample.
A drug screening method comprising:
- (a) providing a microarray chip, wherein the microarray chip comprises a substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells;
- (b) culturing a target cell in the microwells;
- (c) dispensing a candidate drug into the microwells;
- and
- (d) detecting any binding of the candidate drug to the target cell and any phenotypic change resulting from binding of the candidate drug and the target cells.
A method for analyzing binding of a probe to a sample comprising:
- (a) providing a microarray chip, wherein the microarray chip comprises a substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells;
- (b) applying the probe to the sample in the microwells;
- and
- (c) detecting any binding of the probe to the sample and any phenotypic change resulting from binding of the probe to the sample.
Dependencies are inferred from the claim text.