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US 2010/0167948 A1Published application
MicroRNA Expression Profiling of Cerebrospinal Fluid
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsAnna Krichevsky, Brit Mollenhauer
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Abstract
The present invention is directed to assay methods in which the levels of certain specific microRNAs are determined in the cerebrospinal fluid of a subject. These methods may be used in the diagnosis or monitoring of neurological diseases, especially brain tumors.
Claims26
A method of diagnosing or monitoring a neurological disease in a subject, comprising:
- a) obtaining a test sample of cerebrospinal fluid (CSF) from said subject;
- b) assaying said test sample to determine the concentration or amount of one or more microRNAs;
- c) comparing the concentration or amount determined in step b) with the concentration or amount determined for the same one or more microRNAs in one or more control samples of CSF;
- and
- d) concluding that said subject has said neurological disease, or that said neurological disease has progressed or recurred, if the concentration or amount of said microRNA is at least 25% higher or lower in said test sample than in said one or more control samples.
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A microarray useful for diagnosing neurological diseases comprising:
- a) a solid support comprising a membrane, glass or plastic dish, plate or slide;
- and
- b) at least 5 distinct polynucleotides, each of which is attached to said solid support at a separate site and each of which hybridizes under stringent conditions to a different microRNA or complement thereof, wherein said microRNA is selected from the group consisting of:
- miR-21;
- miR-17-5p;
- miR-18;
- miR-19;
- miR-20;
- miR-92;
- mir-10a;
- mir10b;
- mir-96;
- mir-182/182*;
- mir-83;
- mir-15 a;
- mir-15b;
- mir-16;
- mir-125b;
- mir-124;
- mir-1;
- mir-7;
- mir-103;
- mir-134;
- mir-137;
- mir-345;
- mir-200a;
- mir-330;
- mir-485-5p ;
- mir-151;
- mir-22;
- mir-181;
- mir-219;
- mir-30;
- mir-128;
- mir-29a;
- mir-29b;
- mir-29c;
- mir-139;
- mir-338;
- mir-324-3p;
- mir-135;
- mir-296;
- mir-467;
- mir-521;
- mir-155;
- mir-26b;
- mir-132;
- and mir-212.
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Dependencies are inferred from the claim text.