Polymorphisms in Genes Affecting Ace-Related Disorders and Uses Thereof
AssigneeThe Ohio State University Research Foundation
InventorsWolfgang Sadee, Andrew D. Johnson, Danxin Wang, Audrey C. Papp
Life of this patent
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Abstract
A method for predicting a subject's risk factors for ACE-related disorders includes detecting the allelic status of one or more polymorphisms in a nucleic acid sample of the subject.
Claims29
A method for predicting a subject's risk factors for an ACE-related disorder, the method comprising detecting the allelic status of one or more polymorphisms in a nucleic acid sample of the subject, wherein the polymorphism is one or more of:
- (i) ACE-associated SNPs rs4290, rs7214530, rs7213516 or combinations thereof;
- or,
- (ii) a SNP in linkage disequilibrium with one or more SNPs listed in (i)
- wherein the allelic status of the polymorphism in the subject is predictive of the subject's risk for having or developing the ACE-related disorder.
A method of screening a subject for a prognostic biomarker of an ACE-related disorder, comprising detecting the allelic status of one or more polymorphisms in a nucleic acid sample of the subject, wherein the polymorphism is one or more of:
- (i) ACE-associated SNPs rs4290, rs7214530, rs7213516 or combinations thereof;
- or,
- (ii) a SNP in linkage disequilibrium with one or more SNPs listed in (i)
- wherein the allelic status of the polymorphism in the subject is predictive of the prognostic outcome of the ACE-related disorder.
A kit comprising an assay for detecting the allelic status of one or more polymorphisms in a nucleic acid sample of a subject, wherein the polymorphism is one or more of:
- (i) ACE-associated SNPs rs4290, rs7214530, rs7213516 or combinations thereof;
- or, (ii) a SNP in linkage disequilibrium with one or more SNPs listed in (i).
-34. (canceled)
A method for identifying susceptibility to cardiovascular disease in a subject, comprising:
- obtaining a sample from a human subject;
- and determining if the sample contains a risk allele of ACE-associated SNPs rs4290, rs7214530, rs7213516 or combinations thereof.
A method of screening a subject for susceptibility to cardiovascular disease, comprising:
- obtaining a sample from a human subject;
- detecting a risk allele of the ACE-associated SNPs rs4290, rs7214530, rs7213516 or combinations thereof;
- and providing an indication of susceptibility to cardiovascular disease based on the detection of the risk allele.
A microarray comprising oligonucleotide probes capable of hybridizing under stringent conditions to one or more nucleic acid molecules having a polymorphic variant sequence at the site encoding ACE-associated SNPs rs4290, rs7214530, rs7213516 or combinations thereof.
A system for identifying a phenotype for an organism or biological sample derived therefrom, the system comprising:
- a) a set of marker probes or primers configured to detect at least one allelic status associated with the phenotype, wherein the allele comprises ACE-associated SNPs rs4290, rs7214530, rs7213516 or combinations thereof;
- b) a detector that is configured to detect one or more signal outputs from the set of marker probes or primers, or an amplicon produced from the set of marker probes or primers, thereby identifying the presence or absence of the allele;
- and,
- c) system instructions that correlate the presence or absence of the allele with the predicted phenotype, thereby identifying the phenotype for the organism or biological sample derived therefrom.
A subset of SNPs comprising at least two of the ACE-associated SNPs rs4290, rs7214530 and rs7213516.
A composition comprising a set of probes that hybridize to at least two of the ACE-associated SNPs rs4290, rs7214530 and rs7213516.
Dependencies are inferred from the claim text.