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US 2010/0167941 A1Published application
Particle based assay system
OwnerNigel G. Skinner, Paul Fenton +1inventors
InventorsNigel G. Skinner, Paul Fenton, George A. Adaniya
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OwnerNigel G. Skinner, Paul Fenton +1inventors
InventorsNigel G. Skinner, Paul Fenton, George A. Adaniya
Estimating the term…
The invention provides a particle comprising a surface, wherein a portion of the surface is capable of emitting electromagnetic radiation and another portion of the surface is capable of emitting a differential electromagnetic radiation (either different intensity, different frequency or no radiation), and wherein the arrangement of said portions of the surface defines a spatially distributed code for identifying the particle. The invention also provides method of manufacturing a particle having an identifying code comprising providing a particle with a functionalized surface which comprises functional binding moieties and selectively removing a plurality of the functional binding moieties from the surface to create a pattern of functionalized and differentially-functionalized zones on the surface. Various liquid-based assay methods employing the particle of the invention and a kit comprising the particle of the invention are also disclosed.
A particle comprising a surface, wherein a portion of the surface or sub-surface is capable of emitting a first electromagnetic radiation and another portion of the surface is capable of emitting a differential electromagnetic radiation, and wherein the arrangement of said portions of the surface defines a spatially distributed code for identifying the particle.
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A particle comprising a surface wherein the surface comprises at least one modified portion comprising a plurality of functional binding moieties and at least one unmodified portion which is substantially free of functional binding moieties, wherein the modified portion(s) further comprise an electromagnetic radiation emitting species, and wherein the arrangement of modified and unmodified portions on the surface forms a pattern amenable to detection.
A method of manufacturing a particle having an identifying code comprising providing a particle with a functionalized surface which comprises functional binding moieties and selectively removing a plurality of the functional binding moieties from the surface to create a pattern of functionalized and differentially-functionalized zones on the surface.
A method of manufacturing a particle having an identifying code comprising providing a particle having a differentially-functionalized surface and selectively adding a plurality of functional binding moieties to the surface to create a pattern of functionalized and differentially-functionalized zones on the surface.
An article of manufacture having a substantially cylindrical shape with a diameter between about 5 μm and 200 μm, and a length between about 10 μm and 2000 μm, wherein the surface of the particle comprises at least one substantially circumferential functionalized zone, at least one substantially circumferential differentially-functionalized zone between the end of the particle and the functionalized portion or between functionalized portions, and a combination of the width(s) of the functionalized portion(s) and the width(s) of the differentially-functionalized zone(s) establishes a code for identifying the particle.
An assay for the detection of binding between a probe and a target comprising contacting the probe with a solution suspected of containing the target, wherein the probe is attached to a particle which emits electromagnetic radiation upon binding between probe and target and emits electromagnetic radiation as a means of identifying the particle in solution.
Dependencies are inferred from the claim text.