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OwnerRaymond Coker, Olena Piletska +1inventors
InventorsRaymond Coker, Olena Piletska, Sergey Piletsky
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Polymers suitable for binding aflatoxins for use for in solid phase extraction of aflatoxins and immobilisation of aflatoxins in solid phase extraction (SPE) cartridges for qualitative or quantitative analysis of aflatoxins in solutions extracted from foodstuffs or feedstuffs are prepared from allylamine; methylene bisacrylamide (MBAA); acrylamide, ethyleneglycol-methacrylate phosphate (EGMP); 4-vinyl pyridine; or 2-acrylamido-2-methylpropane-sulfonic acid (AMPSA) monomers. Preferred embodiments are polymers containing acrylamide moieties such as acrylamide and MBAA. The polymers are preferably cross-linked, for example using ethylene glycol dimethacrylate (EGDMA) or divinyl benzene (DVB), and macroporous.
Use of a polymer containing moieties derived from an acrylamide based monomer as an SPE adsorbent for aflatoxins.
Use of a cross-linked polymer derived from methylene bisacrylamide (MBAA), allylamine, ethylene glycol methacrylate phosphate (EGMP), 4-vinyl pyridine or 2-acrylamido-2-methylpropane-sulfonic acid (AMPSA) as an SPE adsorbent for aflatoxins.
A fluorometric analysis method for aflatoxins which comprises adsorbing the aflatoxins on a polymer derived from an acrylamide based monomer loaded in or on a cuvette, cartridge, needle, membrane, rod or flat surface, exposing the immobilised aflatoxins to UV light and detecting the fluorescence emitted by the immobilised aflatoxin.
A clean-up method prior to quantitative measurement of aflatoxins, in which a sample liquid is contacted with a polymer derived from an acrylamide based monomer loaded in or on a cuvette, cartridge, rod, needle, membrane or flat surface to adsorb any aflatoxins that are present, and the aflatoxins are eluted from the polymer for quantitative measurement of any aflatoxins present.
An SPE adsorbent unit comprising a cuvette, cartridge, needle, membrane, rod or flat surface loaded or coated with a layer of methylene bisacrylamide (MBBA) cross-linked with ethylene glycol dimethacrylate (EGDMA) as polymer adsorbent.
Dependencies are inferred from the claim text.