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US 2010/0167405 A1Published application
Processes for improved strain engineering
AssigneeNature Technology Corporation
InventorJames A. Williams
Life of this patent
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Abstract
Improvements in strain engineering technology are needed to insure the economic feasibility of future engineered recombinant organisms for industrial biotechnology. Disclosed herein are rapid, efficient methods (Genome Mass Transfer) that facilitate introduction of new selectable traits into a target microbial host. In one preferred embodiment, methods for high efficiency electroporation mediated transfer of donor DNA into a recipient microbial cell are disclosed.
Claims12
A method for engineering microbial cells comprising the steps of:
- a. isolating genomic DNA from a donor strain;
- and
- b. introduction of donor DNA into a recipient strain engineered to express recombineering genes;
- and
- c. selecting for acquisition of a trait in the recipient host;
- whereby said method increases the frequency of targeted incorporation of said donor DNA into said recipient host's genome.
3 more elements
8) A method for engineering cells comprising the steps of:
- a. isolating genomic DNA from a donor strain;
- and
- b. introduction of donor DNA into a recipient strain of the same or different species, which said recipient strain is engineered to express recombineering genes;
- and
- c. selecting for acquisition of a trait in the recipient host;
- whereby said method increases the frequency of targeted incorporation of said donor DNA into said recipient host's genome.
3 more elements
Dependencies are inferred from the claim text.