US 2010/0167397 A1Published application

Endocrine cell lines and method of using the same

AssigneeKyowa Hakko Kogyo Co., Ltd.

InventorsKATSUTOSHI SASAKI, KAZUMI MIURA, SATOSHI SAEKI, MISAKO SUZUKI, KAZUYA KISHIMOTO, HIROFUMI KUNITOMO, TATSUNARI NISHI, MASUO OBINATA

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Abstract

In accordance with the present invention, there are provided (1) various cell lines derived from hypothalamus and Langerhans islets of mammals, (2) process for producing an active peptide and expression cloning system of active peptide precursor gene using the cell line as a host, (3) a method of screening or evaluating a substance capable of acting on the cells using the cell line, (4) a method of screening or isolating a useful gene or useful peptide using the cell line and (5) a highly-sensitive and simple assay system for GPCR ligand used in the above expression cloning system.

Claims10

Dependent claims

A cell line which is obtained by introducing an expression vector haboring a DNA encoding a G-protein coupled receptor, a promoter and a replication origin oriP of Epstein-Barr virus into a B-cell line that is adapted for serum-free culture

wherein the EBNA-1 gene of Epstein-Barr virus is expressed and
wherein at least one of the following (1) to (3) is integrated into a chromosomal DNA;
(1) a DNA construct which expresses a transcription factor necessary for construction of an inducible expression system;
(2) a DNA construct where a reporter gene is ligated downstream of a promoter;
and
(3) a DNA construct for expression of Gα protein or chimeric Gα protein.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application: 12/562,320Filed 18 Sep 2009
Priority: JP 2002-11303016 Apr 2002
Published: 1 Jul 2010
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

G01N 33/507
Involving specific cell types: pancreatic cells
G01N 33/5008
Involving human or animal cells: for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
G01N 33/5023
For testing non-proliferative effects: on expression patterns
G01N 33/5026
For testing non-proliferative effects: on cell morphology

Family

Application10/511,341Continuation · filed 25 Aug 2005
Application12/562,320Continuation
US 2007/0105096 A1Published application
US 2010/0167397 A1This patent

From the US filing history. Foreign family members aren’t listed.

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