Novel Oligonucleotide and NF-kB Decoy Comprising the Same
OwnerToshohiro Nakajima, Akiko Temma +1inventors
InventorsToshohiro Nakajima, Akiko Temma, Naho Suzuki
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Abstract
A novel oligonucleotide useful as an NF-κB decoy having a higher binding ability to NF-κB than known NF-κB decoy as well as the medical uses thereof, is disclosed. The oligonucleotide of the invention has a base sequence having a consensus sequence and specific 5′-flanking and 3′-flanking sequences ligated to both ends of the consensus sequence, respectively. The NF-κB decoy is constituted by an oligonucleotide which is the above-described oligonucleotide of the invention and which is substantially double-stranded wherein the strands are complementary to each other.
Claims17
1-28. (canceled)
An oligonucleotide having a base sequence represented by Formula (I):
- A-X—B (I)
- wherein X is a consensus sequence comprising either gggatttccc (SEQ ID NO:1) or gggactttcc (SEQ ID NO:2);
- A is a 5′-flanking sequence selected from the group consisting of:
- cgc;
- ccc;
- gga;
- cgca;
- ccct;
- and ggct;
- and
- B is a 3′-flanking sequence selected from the group consisting of:
- agc;
- acc;
- ggg:
- gcg;
- gcc;
- and gcgg.
An oligonucleotide decoy for a transcription factor, comprising an oligonucleotide which is substantially double-stranded, wherein the strands in said oligonucleotide are complementary to each other and said oligonucleotide comprises a core sequence and one or more flanking sequences ligated to one or both ends of said core sequence, wherein the bonds between only all of the nucleotides constituting said core sequence are modified by a nuclease-resistant modification and the bonds between all of other nucleotides are not modified.
A method for inhibiting a transcription factor, comprising contacting said transcription factor with an effective amount of an oligonucleotide decoy for the transcription factor, said oligonucleotide decoy comprising an oligonucleotide having a core sequence and one or more flanking sequences ligated to one or both ends of said core sequence, wherein the bonds between all of the nucleotides constituting said core sequence are modified by a nuclease-resistant modification, and the bonds between all of other nucleotides are not modified.
Dependencies are inferred from the claim text.