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AssigneeHawaii Biotech, Inc.
InventorsDavid Edward Clements, Gordon V. L. Wang, Carolyn Weeks-Levy
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The present invention is directed at optimized expression vectors for the expression of native-like heterologous proteins in insect cells. Compositions of the invention are nucleotide sequences representing elements of an expression vector that when combined results in enhanced expression and secretion of heterologous proteins. The elements include sequences that define transcriptional activators, core promoters, secretion signals, and 3′ untranslated regions that are functional in insect cells. The elements contained in the optimized vectors are all synthetically derived or are modified variants of naturally occurring insect sequences. The expression vectors are useful for the expression of native-like proteins when protein encoding nucleotide sequences are operatively linked to the vectors. These vectors can be used to transform insect cells, which can then be cultured to produce the desired protein product. The expressed native-like proteins can be used in diagnostic, vaccine or other applications requiring large amounts of high quality proteins.
An expression vector for expression and secretion of heterologous proteins in cultured insect cells, comprising an expression cassette, wherein the expression cassette contains a synthetic promoter that is composed of proximal and core promoter elements that are operably linked within the expression cassette such that the insertion of a heterologous gene sequence will result in protein expression from cells stably transformed with the expression vector.
An expression vector for expression and secretion of heterologous proteins in cultured insect cells, comprising the expression cassette shown in SEQ. ID. NO:
An expression vector for expression and secretion of heterologous proteins in cultured insect cells, comprising the expression cassette shown in SEQ. ID. NO:
The use of the expression vectors of claims 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 16, 17, 18, 19, 20, or 21, wherein the insect cells are Drosophila cells.
The use of the expression vectors of claims 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 16, 17, 18, 19, 20, or 21, wherein the insect cells are Drosophila S2 cells.
Dependencies are inferred from the claim text.