Back to results
US 2010/0167376 A1Published application
Methods for providing cellular lysates from cell wall-containing samples
AssigneeGenVault Corporation
InventorsMichael Hogan, Michael Saghbini, Joseph Utermohlen, Chunnian Shi
Life of this patent
Estimating the term…
Abstract
The present invention provides methods useful for making lysates from cell wall-containing cellular samples, including plant tissue samples and cultures of yeast or bacteria. The invention further provides compositions (e.g., solutions) that can be used in the methods of the invention, and kits comprising solutions and/or other reagents useful for carrying out the methods of the invention.
Claims38
A method for providing a plant tissue lysate, said method comprising:
- incubating a plant tissue sample in an enzymatic solution,
- wherein said enzymatic solution comprises at least two cell wall and tissue lattice degrading enzyme activities and a metal chelator;
- and
- wherein said enzymatic incubation is carried out in the absence of mechanical processing.
1 more element
A method for providing a plant tissue lysate, said method comprising:
- incubating a plant tissue sample in an enzymatic solution,
- wherein said enzymatic solution comprises at least two cell wall and tissue lattice degrading enzyme activities and a metal chelator;
- and
- wherein said enzymatic incubation is carried out at ambient temperature.
1 more element
A kit comprising:
- a first solution comprising a metal chelator, a preservative, and an ionic detergent, wherein said first solution has a pH of about 4.0 to about 9.0;
- and
- at least two cell wall and tissue lattice degrading enzyme activities.
A mixture comprising:
- plant tissue;
- and
- an enzymatic solution comprising a metal chelator, a preservative, an ionic detergent, and at least two cell wall and tissue lattice degrading enzyme activities,
- wherein the pH of said mixture is about 4.0 to about 9.0.
1 more element
Dependencies are inferred from the claim text.