Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
AssigneeButamax(Tm) Advanced Biofuels LLC
InventorsMichael G. Bramucci, Robert A. Larossa, Dana R. Smulski
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Abstract
Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production.
Claims17
A recombinant yeast cell comprising:
- a) a butanol biosynthetic pathway;
- and
- b) at least one genetic modification which increases activity of the high osmolarity/glycerol response pathway:
- wherein the butanol biosynthetic pathway comprises at least one gene that is heterologous to the yeast cell and wherein the yeast cell has an increase in tolerance to butanol as compared with a yeast cell that lacks the at least one genetic modification of (b).
A recombinant yeast cell comprising a butanol biosynthetic pathway and at least one heterologous PBS2 protein encoding gene.
A method for producing a recombinant yeast cell having increased tolerance to butanol comprising:
- a) providing a recombinant yeast cell comprising a butanol biosynthetic pathway selected from the group consisting of:
- i) a 1-butanol biosynthetic pathway
- ii) a 2-butanol biosynthetic pathway;
- and
- iii) an isobutanol biosynthetic pathway;
- and
- b) engineering the yeast cell of (a) to comprise at least one genetic modification which increases activity of the high osmolarity/glycerol response pathway wherein the genetic modification increases PBS2 mitogen activated protein kinase activity.
A method for improving fermentative production of butanol comprising:
- a) providing a recombinant yeast cell comprising a butanol biosynthetic pathway selected from the group consisting of:
- i) a 1-butanol biosynthetic pathway
- ii) a 2-butanol biosynthetic pathway;
- and
- iii) an isobutanol biosynthetic pathway;
- wherein said yeast cell also comprises at least one genetic modification that increases activity of the high osmolarity/glycerol response pathway and wherein the genetic modification increases PBS2 mitogen activated protein kinase activity;
- and
- b) contacting said yeast cell with fermentable sugar whereby said yeast cell produces butanol and said yeast cell has improved tolerance to said butanol as compared to a yeast cell without at least one genetic modification that increases activity of the high osmolarity/glycerol response pathway and increases PBS2 mitogen activated protein kinase activity.
Dependencies are inferred from the claim text.