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US 2010/0167358 A1Published application
Compositions and processes for improved plasmid dna production
AssigneeNature Technology Corporation
InventorJames A. Williams
Life of this patent
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Abstract
Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity. These processes feature RNA based inducers of plasmid copy number.
Claims11
A method for production of covalently closed super-coiled plasmid DNA comprising the steps of:
- A) growing the bacterial cells containing a plasmid, cosmid, or bacterial artificial chromosome replicon;
- and B) increasing plasmid replication by induction of anti-RNAI;
- and C) continuing growth to accumulate plasmid product;
- whereby said method increases plasmid yield.
1 more element
9) A composition of matter comprising one or more strains of bacteria containing:
- A) at least one anti-RNAI antisense inducer;
- and B) at least one plasmid;
- wherein the anti-RNAI antisense inducer is used to improve the yield of the plasmid obtained from culture.
Dependencies are inferred from the claim text.