Compositions of Aminoacyl-tRNA Synthetase and Uses Thereof
AssigneeAmbrx, Inc.
InventorsAndrew Paulsel, Ho Sung Cho
Life of this patent
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Abstract
Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.
Claims23
1-18. (canceled)
A cell comprising a translation system, wherein the translation system comprises an aminoacyl tRNA synthetase (RS) nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 17, and a complementary polynucleotide sequence of SEQ ID NO:
- 17.
31-35. (canceled)
A vector comprising a polynucleotide encoding an RS having a polynucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 17, and a complementary polynucleotide sequence thereof.
A method of producing a polypeptide in a cell with a selected amino acid at a specified position, the method comprising:
- growing, in an appropriate medium, the cell, where the cell comprises a nucleic acid that comprises at least one selector codon and encodes a polypeptide;
- and,
- providing the selected amino acid;
- wherein the cell further comprises:
- an orthogonal RS (O-RS) that functions in the cell wherein said RS has an amino acid sequence selected from the group consisting of SEQ ID NO:
- 17, and a complementary polynucleotide sequence thereof;
- and,
- an orthogonal tRNA (O-tRNA), that recognizes the selector codon wherein said O-RS aminoacylates the O-tRNA with the selected amino acid.
42-43. (canceled)
A polynucleotide having a polynucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 17, and a complementary polynucleotide sequence thereof.
Dependencies are inferred from the claim text.