Method of producing heterogeneous protein
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHisahiro Tabuchi, Tomoya Sugiyama
Life of this patent
Estimating the term…
Abstract
The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.
Claims12
A method of producing a desired polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding the desired polypeptide and thereby allowing the cell to produce said desired polypeptide.
A cell which has a transferred DNA encoding alanine aminotransferase and a transferred DNA encoding a desired polypeptide.
A cell which has a transferred DNA encoding alanine aminotransferase and a transferred DNA encoding a taurine transporter.
A method of producing a desired polypeptide, comprising culturing in an α-ketoglutarate-containing medium a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding the desired polypeptide and thereby allowing the cell to produce said desired polypeptide.
Dependencies are inferred from the claim text.