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AssigneeKyoto University
InventorsShigeru Amano, Yoshinobu Toda
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A cassette for fixing, embedding and slicing biological tissues includes a base sheet and a lid sheet formed of an elongated plastic sheet which is sliceable and has not less than predetermined strength. The base sheet has grooves as biological tissue receiving portions. The base plate and the lid sheet are formed by folding the plastic sheet along a fold line so that the lid sheet can be selectively opened and closed. Biological tissues are pressed against the bottoms of the grooves with pressing/retaining sponge members secured to the lid sheet at its portions corresponding to the respective grooves. In this state, the biological tissues are fixed by a fixing agent, and the cassette is dehydrated, infiltrated with paraffin as an embedding agent to embed the cassette, thereby forming a block including an embedding frame. The block is then sliced with a microtome to provide good biological tissue specimens.
A cassette for fixing, embedding and slicing biological tissues, said cassette comprising a base plate 11 formed of a sliceable blank plate which has hardness and strength both higher than predetermined levels and on which biological tissues can be placed, a lid plate 12 formed by bending the blank plate forming the base plate, or formed of a separate blank plate and detachably coupled to the base plate, said base plate 11 having a tissue receiving portion 13B for receiving biological tissues, and a pressing/retaining member 13A detachably coupled to the lid plate 12 or provided separately from the lid plate 12 for immovably pressing and retaining the biological tissues, said base plate and said lid plate being formed with numerous small holes 16.
Dependencies are inferred from the claim text.