Method for cleavage of peptidic bond at c terminal of peptide and a method for determination of c terminal amino acid sequence of peptide

A method for determination of C terminal amino acid sequence of peptide comprising the steps of:

• reacting a peptide with a solution of perhalogenated carboxylic acid containing alcohol to esterify glutamic acid residue of said peptide;
• reacting said peptide with alkanoic acid anhydride to obtain C terminal-deleted peptide in which the amino acid residue of said peptide at C terminal is sequentially deleted;
• measuring molecular weight of said C terminal-deleted peptide;
• and
• grasping said peptide based on said molecular weight.

A method for determination of C terminal amino acid sequence of peptide comprising the steps of:

• reacting a peptide with a solution of perhalogenated carboxylic acid containing alcohol to esterify glutamic acid residue of said peptide;
• reacting said peptide with alkanoic acid anhydride to obtain C terminal-deleted peptide in which the amino acid residue of said peptide at C terminal is sequentially deleted;
• reacting said peptide with site specific cleaving agent to obtain a C terminal-deleted peptide originated peptide fragment originated from said C terminal-deleted peptide;
• measuring molecular weight of said C terminal-deleted peptide originated peptide fragment;
• and
• grasping said peptide based on said molecular weight.

A method for determination of C terminal amino acid sequence of peptide supported in a substrate comprising the steps of:

• reacting a peptide supported in a substrate with a solution of perhalogenated carboxylic acid containing alcohol to esterify glutamic acid residue of said peptide;
• reacting said peptide with alkanoic acid anhydride to obtain C terminal-deleted peptide in which the amino acid residue of said peptide at C terminal is sequentially deleted;
• immersing said substrate into a solution containing site specific cleaving agent to obtain a C terminal-deleted peptide originated peptide fragment originated from said C terminal-deleted peptide;
• measuring molecular weight of said C terminal-deleted peptide originated peptide fragment;
• and
• grasping said peptide based on said molecular weight.