Epitope/peptide recognized by hla-a2402-restricted ep-cam-specific ctl and use of the same

A method of quantifying HLA-A2402-restricted cytotoxic T lymphocytes in peripheral blood, comprising

• making an antigen-presenting cell act on peripheral blood, wherein the antigen-presenting cell was pulsed with an isolated peptide of either (1) or (2):
• (1) a peptide consisting of the amino acid sequence represented by SEQ ID NO:
• 1
• (2) a peptide consisting of the amino acid sequence represented by SEQ ID NO:
• 2, and
• quantifying cytotoxic T lymphocytes in peripheral blood or cytokine produced by such cytotoxic lymphocytes.

A method of quantifying HLA-A2402-restricted cytotoxic T lymphocytes in peripheral blood, comprising steps (i) and (ii)

• (i) making a major histocompatibility antigen complex act on peripheral blood,
• wherein the major histocompatibility antigen complex comprises a major histocompatibility antigen, and an isolated peptide or a tumor antigen epitope peptide present on an antigen-presenting cell,
• the isolated peptide is either (1) or (2):
• (1) a peptide consisting of the amino acid sequence represented by SEQ ID NO:
• 1
• (2) a peptide consisting of the amino acid sequence represented by SEQ ID NO:
• 2, and
• the antigen-presenting cell was pulsed with said isolated peptide, and
• (iii) quantifying cytotoxic T lymphocytes in peripheral blood or cytokine produced by such cytotoxic lymphocytes.

A method of quantifying HLA-A2402-restricted cytotoxic T lymphocytes in peripheral blood, comprising steps (i) and (ii)

• (i) making a major histocompatibility antigen complex tetramer act on peripheral blood, wherein the major histocompatibility antigen complex tetramer comprises a major histocompatibility antigen and an isolated peptide or the tumor antigen epitope peptide present on the antigen-presenting cell, the isolated peptide is either (1) or (2):
• (1) a peptide consisting of the amino acid sequence represented by SEQ ID NO:
• 1
• (2) a peptide consisting of the amino acid sequence represented by SEQ ID NO:
• 2, and
• the antigen-presenting cell was pulsed with said isolated peptide, and
• (ii) quantifying cytotoxic T lymphocytes in peripheral blood or cytokine produced by such cytotoxic lymphocytes.