Compositions and Methods for Detecting and Quantifying Toxic Substances in Disease States

A method of detecting an abnormal condition in a subject, said method comprising

• a) detecting the binding activity of a binding agent towards at least one standard to establish a standard curve, said standard comprising a synthetic aggregated peptide (SAP);
• b) contacting a sample from said subject with at least one binding agent that is capable of binding a biomarker, wherein said biomarker is an aggregated biomarker;
• c) detecting the level binding activity of said binding agent in said sample;
• d) correlating said level of binding activity in said sample to said standard curve to determine the levels of said aggregated biomarker in said subject;
• and
• e) comparing the levels of said aggregated biomarker in said subject to normal levels of said aggregated biomarker to determine a difference between measured levels of said aggregated biomarker and normal levels of said aggregated biomarker;
• wherein a difference between said measured levels of said aggregated biomarker and said normal levels of said aggregated biomarker, is indicative of an abnormal condition in said subject.