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US 2010/0167303 A1Published application
Sequences diagnostic for shrimp pathogens
AssigneeE. I. Du Pont de Nemours and Company
InventorsRICHARD C. EBERSOLE, Jianzhong Zhang, Mario W. Chen, Christian Peter Lenges
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Abstract
Primers have been isolated that are diagnostic for the detection of Vibrio harveyi. The primers are based on a portion of the Vibrio harveyi LuxR gene and may be used in primer directed amplification or nucleic acid hybridization assay methods.
Claims7
A method for detecting the presence of Vibrio harveyi in a sample comprising:
- (i) providing DNA from a sample suspected of containing Vibrio harveyi;
- and
- (ii) probing the DNA with a probe derived from the isolated Vibrio harveyi diagnostic primer sequence as set forth in SEQ ID NO:1 or SEQ ID NO:2 under suitable hybridization conditions;
- wherein the identification of a hybridizable nucleic acid fragment confirms the presence of Vibrio harveyi.
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Dependencies are inferred from the claim text.