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US 2010/0167291 A1Published application
Assessing expression of endogenous and exogenous genes
AssigneePromega Corporation
InventorsMartin Rosenberg, Douglas Storts
Life of this patent
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Abstract
The present invention relates to compositions and methods to assess gene expression in cells. In particular, the present invention provides compositions and methods to assess expression from an exogenous gene and an endogenous version of the same gene in induced pluripotent stem cells.
Claims18
A method comprising:
- a) providing:
- i) one or more cells;
- ii) an exogenous gene encoding a de-differentiation factor, said exogenous gene differing in sequence from an endogenous version of said gene in said one or more cells;
- b) introducing said exogenous gene into said one or more cells to induce de-differentiation of said one or more cells;
- and
- c) detecting the presence of, absence of, or amount of expression of said exogenous gene and said endogenous gene.
3 more elements
A composition comprising:
- a) a synthetic, non-native de-differentiation gene having a sequence that differs from a native de-differentiation gene;
- and
- b) a reagent that detects an expression product of said synthetic, non-native de-differentiation gene and differentiates said expression product from an expression of said native de-differentiation gene.
Dependencies are inferred from the claim text.