Methods and Compositions Related to Continuous Flow Thermal Gradient PCR
AssigneeUniversity of Utah Research Foundation
InventorsBruce Kent Gale, Niel Davenport Crews, Carl Thomas Wittwer
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Abstract
Disclosed are compositions and a method for amplification and detection of nucleic acid sequences based on continuous flow thermal gradient PCR.
Claims67
A device for replicating nucleic acid, said device comprising:
- a microchannel extending from an inlet port to an outlet port;
- and a heater or heaters for producing a spatial temperature gradient.
A device for replicating a nucleic acid, said device comprising:
- a microchannel;
- two plates;
- and a heater;
- wherein the microchannel is sandwiched between the two plates and the heater is operable to form a spatial temperature gradient across the microchannel.
A device for replicating nucleic acid, said device comprising:
- a channel having a plurality of sections forming a continuous pattern;
- and a heater disposed along a centerline of the continuous pattern;
- wherein each section of the channel comprises a first portion and a second portion, the first portion of the channel being narrower than the second portion of the channel.
A method of amplifying a nucleic acid, the method comprising the steps of:
- a) forming a steady state temperature gradient on a device comprising microchannels;
- and
- b) exposing a nucleic acid to the temperature gradient in a manner conducive for amplification;
- thereby amplifying a nucleic acid.
A method for monitoring nucleic acid replication using a microchip, said method comprising the steps of:
- a) forming a temperature gradient across a device;
- and b) exposing a nucleic acid to the temperature gradient in a manner conducive for amplification;
- and c) detecting nucleic acid amplification using fluorescent monitoring;
- thereby monitoring nucleic acid amplification using a microchip.
A method of forming a chip with microchannels for use in continuous-flow PCR, said method comprising the steps of:
- a) creating a digital image of the microchannels using a digital computing machine;
- b) sending the digital image of the microchannels to a plotting device such that the plotting device forms the microchannels on a thin film;
- and c) sandwiching the thin film between two plates;
- thereby forming a chip with microchannels for use in continuous-flow PCR.
Dependencies are inferred from the claim text.