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US 2010/0167276 A1Published application
High-throughput methods for quantifying cells in environmental and laboratory samples
AssigneeSan Diego State University Research Foundation
InventorsForest Rohwer, Linda Wegley
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Abstract
The invention provides compositions (e.g., kits) and methods for determine the number of bacteria and other microbes in samples having low concentrations of microbes, for use, e.g., in biological warfare defense, microbe detection and agricultural and environmental sciences.
Claims12
A method for enumerating (counting) microbial cells in a sample, the method comprising the following steps:
- (i) (a) providing a liquid or water sample, and optionally, if appropriate, making a solution comprising an initial non-liquid sample;
- (b) filtering the liquid or water sample with at least one low protein binding filter;
- (c) adding SYBERGOLD™ gold nucleic acid stain, or equivalent;
- (d) measuring the total fluorescence, thereby enumerating (counting) microbial cells in the sample;
- (ii) the method of (i), wherein the sample is an environmental sample;
- (iii) the method of (ii), wherein the environmental sample comprises a biological, soil, air or water sample;
- (iv) the method of claim (iii), wherein the water sample comprises water from a salt water, hot spring, public water supply, water tank, reservoir, fresh water, aquifer, storm drain, river, lake or aquaculture pond water source;
- or
- (v) the method of claim (iii), wherein the biological sample comprises a plant, seed or an animal tissue sample.
6 more elements
2-5. (canceled)
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Dependencies are inferred from the claim text.