Methods and kits for identifying nucleic acid sequences
OwnerJoel Stavans, Dror Sagiinventors
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Abstract
Methods and kits for detecting a nucleic acid sequence-of-interest in a sample are provided. The methods and kits use RecA-mediated strand exchange which is sensitive to a single-mismatch and which can exchange strands of non-denatured polynucleotide sequences of a sample. Thus the methods and kits of the present invention can be used for the identification of single nucleotide polymorphisms (SNPs), length of telomeric ends, numeration of chromosomes and disease-associated genes in a sample.
Claims29
A method of detecting a nucleic acid sequence-of-interest in a sample, the method comprising:
- (a) providing a complex of a polynucleotide sequence of the sample and a recombinase;
- (b) incubating said complex with a labeled polynucleotide which comprises a nucleic acid sequence complementary to the nucleic acid sequence-of-interest under conditions suitable for exchange between said polynucleotide sequence and said labeled polynucleotide;
- and
- (c) measuring a rate and/or amount of said exchange to thereby detect the nucleic acid sequence-of-interest in the sample.
A kit for detecting a nucleic acid sequence-of-interest in a sample, the kit comprising packaging materials and at least one agent identified by said packaging material as being suitable for measuring a rate and/or amount of a recombinase-mediated exchange between a polynucleotide sequence of the sample and a labeled polynucleotide which comprises a nucleic acid sequence complementary to the nucleic acid sequence-of-interest.
A method of detecting a nucleic acid sequence-of-interest in a sample, the method comprising:
- (a) providing a complex of a labeled polynucleotide which comprises a nucleic acid sequence complementary to the nucleic acid sequence-of-interest and a recombinase and;
- (b) incubating said complex with the sample under conditions suitable for exchange between said labeled polynucleotide and the polynucleotide sequence of the sample;
- and
- (c) measuring a rate and/or amount of said exchange to thereby detect the nucleic acid sequence-of-interest in the sample.
A kit for detecting a nucleic acid sequence-of-interest in a sample, the kit comprising packaging materials and at least one agent identified by said packaging material as being suitable for measuring a rate and/or amount of a recombinase-mediated exchange between a labeled polynucleotide which comprises a nucleic acid sequence complementary to the nucleic acid sequence-of-interest and a polynucleotide sequence of the sample.
(canceled)
Dependencies are inferred from the claim text.