Seroconversion assays for detecting xenotropic murine leukemia virus-related virus
OwnerJudy A. Mikovits, Francis W. Ruscetti +1inventors
InventorsJudy A. Mikovits, Francis W. Ruscetti, Sandra K. Ruscetti
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Abstract
Methods of detecting, diagnosing, monitoring or managing an XMRV-related disease such as an XMRV-related neuroimmune disease such as chronic fatigue syndrome or an XMRV-related lymphoma such as mantle cell lymphoma in a subject are disclosed. These methods comprise determining presence, absence or quantity of antibodies against XMRV in a sample from a subject.
Claims108
A method of detecting presence, absence or quantity of antibody against XMRV in a subject, the method comprising:
- providing a biological sample comprising antibody from the subject;
- forming a mixture comprising a) at least one gammaretrovirus antigen, b) the sample, and c) a competitive probe against the at least one Gammaretrovirus antigen, under conditions sufficient for formation of a complex comprising the at least one Gammaretrovirus antigen and the competitive probe;
- and
- detecting quantity of a complex comprising the at least one Gammaretrovirus antigen and the competitive probe, whereby if the sample comprises antibody against the Gammaretrovirus antigen, the quantity of complex is less than that of a complex formed from a mixture comprising a) the at least one Gammaretrovirus antigen, b) a control sample not comprising antibody against the at least one Gammaretrovirus antigen, and c) the competitive probe against the at least one Gammaretrovirus antigen.
A method of detecting, diagnosing, monitoring or managing an XMRV-related disease in a subject, the method comprising:
- providing a biological sample comprising antibody from the subject;
- forming a mixture comprising a) at least one Gammaretrovirus antigen and b) the sample, under conditions sufficient for formation of a complex comprising the at least one Gammaretrovirus antigen and antibody against the at least one Gammaretrovirus antigen if present in the antibody from the subject;
- and
- detecting presence, absence or quantity of a complex comprising the at least one Gammaretrovirus antigen and antibody against the at least one Gammaretrovirus antigen.
Dependencies are inferred from the claim text.