Apparatus and method for the preservation of pancreatic tissue and islet cells for transplantation
AssigneeBaylor Research Institute
InventorsShinichi Matsumoto, Tetsuya Ikemoto
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Abstract
The present invention includes compositions and methods for the preparation, preservation and storage of organs (e.g., pancreatic islet cells) for transplantation by storing organs in which the organs are suspended in a solution for maintaining viability and the organ or cells are cooled in a refrigeration unit for the entire duration of storage in which the average temperature in the apparatus does not vary by more than 2 degrees centigrade from the set temperature.
Claims37
An apparatus for storing organs, tissues, or cells in which the organs, tissues, or cells are suspended in a solution for maintaining viability and the organs, tissues, or cells are cooled in a refrigeration unit for the entire duration of storage in which the average temperature in the apparatus does not vary by more than 2 degrees centigrade from the set temperature.
A method of preserving an organ or tissue comprising:
- obtaining an organ or tissue for transplant;
- placing the organ or tissue in an preservation solution;
- cooling the organ or tissue to a pre-selected temperature;
- and
- maintaining the organ or tissue at the pre-selected temperature for the duration of storage at a temperate that does not vary more that 2 degrees centigrade from the pre-selected temperatures.
A method of preparing a transplantable islet preparation, the method comprising the steps of:
- harvesting a pancreas or a pancreatic tissue from a donor;
- injecting one or more pancreatic ducts with ET-Kyoto solution or equivalent thereto;
- isolating pancreatic β-islet cells;
- cooling the pancreas or pancreatic tissue to a pre-selected temperature;
- and
- maintaining the pancreas or pancreatic tissue at the pre-selected temperature in an apparatus for the duration of storage at a temperate that does not vary more that 2 degrees centigrade from the pre-selected temperatures.
A method of storing a transplantable organ or tissue, the method comprising the steps of:
- harvesting a transplantable organ or tissue;
- placing the organ or tissue in a preservation solution;
- cooling the organ or tissue to a pre-selected temperature;
- and
- maintaining the organ or tissue at the pre-selected temperature for the duration of storage at a temperate that does not vary more that 1 degree centigrade from the pre-selected temperatures.
Dependencies are inferred from the claim text.