High potency recombinant antibodies, methods for producing them and use in cancer therapy
AssigneeMedlmmune Way
InventorsPartha Chowdhury, Herren Wu, Laura Richman
Life of this patent
Estimating the term…
Abstract
The present invention contemplates improved recombinant anti-tumor antibodies having faster Kon and faster Koff rates, resulting in a uniform tumor penetrance, as compared to the same recombinant anti-tumor antibody without said faster Kon and faster Koff rates, and methods of improving the same.
Claims21
A method of improving a recombinant anti-tumor antibody's penetrance into a targeted tumor, comprising:
- (a) increasing the Kon rate by at least 2 fold as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate;
- and
- (b) achieving a uniform tumor penetrance by said improved recombinant anti-tumor antibody into said targeted tumor as measured by immunohistochemistry staining (IHC) as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate and demonstrates a non-uniform or patchy staining as measured by IHC.
-4. (canceled)
-8. (canceled)
A method of improving a recombinant anti-tumor antibody's penetrance into a targeted tumor, comprising:
- (a) increasing the Kon rate by at least 2 fold as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate;
- and
- (b) achieving an intra-tumoral diffusion greater than 3 tumor cell diameters as measured by IHC, as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate and demonstrates an intra-tumoral diffusion of 3 or less tumor cell diameters as measured by IHC.
(canceled)
-22. (canceled)
A recombinant anti-tumor antibody having improved tumor penetrance into a targeted tumor, produced by a method comprising:
- (a) increasing the Kon rate by at least 2 fold as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate;
- and
- (b) achieving a uniform tumor penetrance by said improved recombinant anti-tumor antibody into said targeted tumor as measured by immunohistochemistry staining (IHC) as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate and demonstrates a non-uniform or patchy staining as measured by IHC.
A recombinant anti-tumor antibody having improved tumor penetrance into a targeted tumor, produced by a method comprising:
- (a) increasing the Kon rate by at least 2 fold as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate;
- and
- (b) achieving an intra-tumoral diffusion greater than 3 tumor cell diameters as measured by IHC, as compared to the same recombinant anti-tumor antibody without such an increase in Kon rate and demonstrates an intra-tumoral diffusion of 3 or less tumor cell diameters as measured by IHC.
-50. (canceled)
Dependencies are inferred from the claim text.