Uses of thioredoxin
OwnerGarth Powis, Lynn Kirkpatrickinventors
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Abstract
The present invention relates to the use of thioredoxin as, inter alia, a cell growth stimulator, as well as a screen for agents that are useful in reducing or preventing thioredoxin-associated apoptosis inhibition and agents that are useful in inhibiting thioredoxin stimulated cell growth.
Claims8
A method of inhibiting tumor cell growth in a tumor cell that over-expresses thioredoxin comprising contacting said tumor cell with a cell growth inhibiting effective amount of an inhibitor of thioredoxin expression.
A method of reducing inhibition of apoptosis in tumor cells that over-express thioredoxin comprising contacting said tumor cells with an effective amount of an agent that inhibits thioredoxin.
A method of identifying an agent that inhibits tumor cell growth in cells that over-express thioredoxin comprising
- measuring thioredoxin expression in a first sample of said cells;
- contacting a second sample of said cells with an agent to be tested;
- measuring expression of thioredoxin in said second sample;
- comparing expression of thioredoxin in said first sample and said second sample;
- whereby a decrease in expression of thioredoxin in said second sample is indicative of an agent that inhibits tumor cell growth.
A method of identifying an agent that reduces inhibition of apoptosis in a tumor cell that over-expresses thioredoxin comprising
- measuring thioredoxin expression in a first sample of said cells;
- contacting a second sample of said cells with an agent to be tested;
- measuring expression of thioredoxin in said second sample;
- comparing expression of thioredoxin in said first sample and said second sample;
- whereby a decrease in expression of thioredoxin in said second sample is indicative of an agent that reduces inhibition of apoptosis.
A method of identifying an agent that reduces inhibition of apoptosis in a tumor cell growth.
A method of stimulating cell growth comprising introducing a nucleic acid encoding a human thioredoxin having Ser at amino acid reside 73 under conditions whereby said nucleic acid is expressed.
A composition comprising an agent that is useful in reducing or eliminating thioredoxin-associated apoptosis inhibition and an acceptable carrier.
A composition comprising an agent that is useful in inhibiting thioredoxin stimulated cell growth and an acceptable carrier.
Dependencies are inferred from the claim text.