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AssigneeAthenix Corporation
InventorsCheryl L. Peters, Brian Vande Berg, Brian Carr, Julia T. Daum, Vadim Beilinson, Sandra Volrath, Candace Poutre, Kimberly Sampson, Theodore Kahn
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Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence having nematicidal activity, wherein said nucleotide sequence is selected from the group consisting of:
A transgenic plant cell comprising a nucleotide sequence encoding a heterologous polyphenol oxidase, wherein a plant or seed regenerated from said transgenic cell has nematicidal activity.
An isolated polypeptide with nematicidal activity, selected from the group consisting of:
A method for killing or controlling a nematode pest, comprising contacting said pest with, or feeding to said pest, a nematicidally-effective amount of a composition comprising a polyphenol oxidase enzyme.
A method for protecting a plant from a pest, comprising introducing into said plant or cell thereof at least one expression vector comprising a nucleotide sequence that encodes a nematicidal polyphenol oxidase polypeptide, wherein said plant is planted in an area susceptible to nematode infestation.
Dependencies are inferred from the claim text.