Loading patent…
AssigneeThe General Hospital Corporation
InventorsKenneth R. Chien, Leslie Caron, Atsushi Nakano, Alessandra Moretti
Estimating the term…
The present invention relates to isolation of cardiovascular stem cells, and more particularly to cardiovascular stem cells positive for markers isll+/Nkx2.5+/flkl+ and cardiovascular stem cells which can differentiate along endothelial, cardiac, and smooth muscle cell lineages. The invention relates to uses of the cardiovascular stem cells, in particular for the treatment of cardiovascular disorders and as an assay comprising a plurality of cardiovascular stem cells. The invention also relates to a method for isolation and enrichment of stem cells using mesenchymal cell feeder layer and uses of mesenchymal feeder layer as a screening assay for agents which effect stem cells.
A method for isolating cardiovascular stem cells, the method comprising contacting a population of cells with agents reactive to Islet1, Nkx2.5 and flk1, and separating reactive positive cells from non-reactive cells.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
-28. (canceled)
A composition comprising an isolated population of Islet1+, Nkx2.5+ and flk1+ cardiovascular stem cells.
-130. (canceled)
-138. (canceled)
-162. (canceled)
Dependencies are inferred from the claim text.