Method and system for microfluidic device and imaging thereof

A microfluidic system, the system comprising:

• a rigid substrate material, the rigid substrate material having a surface region, the surface region being capable of acting as a handle substrate;
• a deformable fluid layer coupled to the surface region;
• one or more well regions formed in a first portion of the deformable fluid layer, the one or more well regions being capable of holding a fluid therein;
• one or more channel regions formed in a second portion of the deformable fluid layer, the one or more channel regions being coupled to one or more of the well regions;
• an active region formed in the deformable fluid layer, the active region including the one or more well regions;
• a non-active region formed in the deformable fluid layer, the non-active region being formed outside of the first portion and the second portion;
• a first fiducial marking formed within the non-active region and disposed in a spatial manner associated with at least one of the channel regions;
• a second fiducial marking formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions;
• a control layer coupled to the fluid layer, the control layer including one or more control regions;
• and
• a third fiducial marking formed within the control layer.