Method for Counting Colonies

Abstract

Problem to be Solved There is provided a colony counting method capable of obtaining highly reliable and stable colony count values with easy and simple means by eliminating the variation in colony count due to image capturing conditions when the number of colonies is counted by performing data processing on an image obtained by image capturing means performing image capturing on a petri dish. Solution When the number of colonies is counted by performing image capturing and data processing on microbial colonies developed in a culture medium 1 in a petri dish 2 by a CCD camera 7 (image capturing means) provided in an extension direction of a rotational axis C1, which is set near a center of the culture medium surface if of the petri dish, the petri dish is rotated around the rotational axis by a specified angle obtained by dividing one rotation of the petri dish by a predetermined number, and image data of the entire culture medium surface of the petri dish is acquired for each rotation by a specified angle; the number of colonies at each specified angle is counted separately by performing data processing on image data of the entire culture medium surface of the petri dish at the specified angle; and the number of colonies in the petri dish is calculated by performing numerical processing on the number of colonies counted separately at each specified angle.

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