Electrodic bridge
AssigneeGE Healthcare Bio-Sciences AB
InventorsBengt Bjellqvist, Kristina Uhlen
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Abstract
The present invention relates to an electrode bridge or sample application bridge for use in electrophoresis, preferably isoelectric focusing, IEF. More closely, the invention relates to an electrodic bridge or sample loading bridge for preventing denaturant depletion from the IPG (immobilised pH gradient) gel and, optionally, for loading samples onto the IPG gel. The pH of the bridge is adjustable for adoption to positioning at the acid as well as basic end of the IPG strips.
Claims12
An electrode bridge for positioning between an anodic or cathodic electrode and one end of an IPG (Immoblised pH gradient) gel for isoelectric focusing, wherein the electrode bridge is provided with denaturant and pH-adjusting groups, and wherein, for an anodic bridge, the pH after finished focusing is equal or more acidic than the lowest pH of the IPG-gel, and wherein, for a cathodic bridge, the pH after finished focusing is equal or more basic than the highest pH of the IPG-gel.
(canceled)
Dependencies are inferred from the claim text.