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AssigneesDepartment of Biotechnology (Dbt), All India Institute of Medical Sciences (Aiims)
InventorsBiplab Bose, Subrata Sinha, Navin Khanna, Subrat Kumar Acharya
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The invention relates to a recombinant chimeric Fab antibody comprising a recombinant Fd and a recombinant chimeric light chain which binds to hepatitis B surface antigen with high affinity, generated by fusing variable region genes (VH and VL) of an anti-HBsAg mouse antibody 5S and constant region genes of human, CH1 region of human IgGI and the CL region of human kappa chain, wherein mouse VL and human CL are linked by overlap PCR to generate chimeric light chain and mouse VH and CH1 are linked by overlap PCR to generate chimeric Fd.
A recombinant chimeric Fab antibody comprising a recombinant Fd and a recombinant chimeric light chain which binds to hepatitis B surface antigen with high affinity, generated by fusing variable region genes (VH and VL) of an anti-HBsAg mouse antibody 5S and constant region genes of human, CH1 region of human IgGI and the CL region of human kappa chain, wherein mouse VL and human CL are linked by overlap PCR to generate chimeric light chain and mouse VH and CH1 are linked by overlap PCR to generate chimeric Fd.
A recombinant chimeric Fab antibody wherein it can be used either on its own or as a starting material for another molecule, with or without appropriate modifications or conjugations for developing a diagnostic reagent for the detection of Hepatitis B surface antigen.
Dependencies are inferred from the claim text.