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US 2010/0144838 A1Published application
Methods for Identifying Modulators of Pyrimidine Tract Binding Protein
OwnerWilliam T. Beck, Xiaolong Heinventors
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Abstract
Provided herein are methods and materials for identifying compounds that modulate polypyrimidine tract binding protein (PTB), a protein that functions as a negative regulator of pre-mRNA splicing by blocking the inclusion of numerous alternative exons into mRNA.
Claims39
A method of screening for a modulator of PTB comprising:
- (a) providing a cell comprising PTB and a reporter system, wherein the reporter system comprises a first PTB target gene operably linked to a reporter sequence;
- (b) contacting the cell with a candidate modulator compound;
- and
- (c) measuring the level of expression of the PTB target gene;
- wherein a modulator of PTB is identified by a change in expression compared to a control.
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Dependencies are inferred from the claim text.