siRNA-mediated gene silencing with viral vectors
AssigneeUniversity of Iowa Research Foundation
InventorsBeverly L. Davidson, Haibin Xia, Qinwen Mao
Life of this patent
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Abstract
The present invention is directed to viral vectors encoding small interfering RNA molecules (siRNA) targeted against a gene of interest, and methods of using these viral vectors.
Claims28
A viral vector comprising an expression cassette, wherein the expression cassette comprises a nucleic acid sequence encoding a small interfering RNA molecule (siRNA) targeted against a gene of interest.
A viral vector comprising an expression cassette, wherein the expression cassette comprises a nucleic acid sequence encoding a first segment, a second segment located immediately 3′ of the first segment, and a third segment located immediately 3′ of the second segment, wherein the first and third segments are each less than 30 base pairs in length and each more than 10 base pairs in length, and wherein the sequence of the third segment is the complement of the sequence of the first segment, and wherein the nucleic acid sequence functions as a small interfering RNA molecule (siRNA) targeted against a gene of interest.
28-55. (canceled)
Dependencies are inferred from the claim text.