Fermentative production of hydroxytyrosol
OwnerJühane Achkar, Abel Ferrandezinventors
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Abstract
The present invention relates to a newly identified microorganisms capable of direct production of hydroxytyrosol (hereinafter also referred to as Hy-T) from a carbon source obtainable from the D-glucose metabolization pathway. The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Hy-T. The invention also relates to genetically engineered microorganisms and their use for the direct production of Hy-T.
Claims13
A process for the fermentative production of Hydroxytyrosol (Hy-T), wherein a transformed host cell is cultivated under suitable culture conditions that allow the direct production of Hy-T from a carbon source obtainable from the D-glucose metabolization pathway and wherein the genome of said host cell is genetically engineered with a polynucleotide encoding an enzyme capable of transforming tyrosol to Hy-T and at least one polynucleotide encoding an enzyme which has an activity selected from the group consisting of:
- phenylacetaldehyde reductase activity,
- aromatic amino acid decarboxylase, for example L-phenylalanine and/or L-tyrosine decarboxylase activity,
- monoamine oxidase activity,
- a lyase activity,
- phenylpyruvate decarboxylase activity,
- monophenol monooxygenase, for example a tyrosinase activity,
- toluene monooxygenase, for example, toluene para-monooxygenase activity,
- phenylalanine-4-hydroxylase and/or pterin-4-alpha-carbinolamine dehydratase activity, and
- chorismate mutase and/or perphenate dehydrogenase activity.
A genetically engineered host cell able to produce hydroxytyrosol from a carbon source obtainable from the D-glucose metabolization pathway, wherein said host cell is genetically engineered with a polynucleotide encoding an enzyme capable of transforming tyrosol to Hy-T and at least one polynucleotide encoding an enzyme which has an activity selected from the group consisting of:
- phenylacetaldehyde reductase activity,
- aromatic amino acid decarboxylase, for example L-phenylalanine and/or L-tyrosine decarboxylase activity,
- monoamine oxidase activity,
- a lyase activity,
- phenylpyruvate decarboxylase activity,
- monophenol monooxygenase, for example a tyrosinase activity,
- toluene monooxygenase, for example, toluene para-monooxygenase activity,
- phenylalanine-4-hydroxylase and/or pterin-4-alpha-carbinolamine dehydratase activity, and
- chorismate mutase and/or perphenate dehydrogenase activity.
Dependencies are inferred from the claim text.