Penicillin-g acylases
AssigneeCodexis, Inc.
InventorsBEHNAZ BEHROUZIAN, ANKE KREBBER, EMILY MUNDORFF
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Abstract
The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
Claims25
An engineered penicillin G acylase (PGA) capable of cleaving substrate 6-nitro-3-(phenylacetamido)benzoic acid (NIPAB) to product 5-amino-2-nitrobenzoic acid, wherein the PGA comprises an α-chain sequence having at least 85% identity to SEQ ID NO:179 and an β-chain sequence having at least 85% identity to SEQ ID NO:180, wherein the polypeptide has improved activity as compared to the naturally occurring PGA of Kluyvera citrophila.
A composition comprising:
- (i) a penicillin G of structural formula (I), a 6-amino penicillanic acid of structural formula (II), and/or a phenylacetic acid of structural formula (III);
- and (ii) an engineered PGA selected comprising an amino acid sequence selected from SEQ ID NO:
- 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, or 178.
Dependencies are inferred from the claim text.